The growth response of rice (Oryza sativa L. var. FARO 44) in vitro after inoculation with bacterial isolates from a typical ferruginous ultisol

Abstract Background Rice forms a significant portion of food consumed in most household worldwide. production has been hampered by soil factors such as ferruginousity which limited phosphorus availability; an important mineral component for the growth and yield rice. The presence phosphate-solubilizing bacteria (PSB) soils reported to enhance phosphate availability. In view this, present study employed three species (BCAC2, EMBF2 BCAF1) that were previously isolated proved P solubilization capacities inocula investigate response rice germinants vitro setup. isolates first identified using 16S rRNA gene sequencing then applied inoculum. inolula prepared concentrations (10, 7.5 5.0 ml) following McFarland standard. Viable (var. FARO 44) seeds sown petri dishes inoculated with at different concentrations. setup was studied 28 days. Results as: isolate BCAC2= Bacillus cereus strain GGBSU-1, BCAF1= Proteus mirabilis TL14-1 EMBF2= Klebsiella variicola AUH-KAM-9. Significant improvement germination, morphology, physiology biomass parameters bacteria-inoculated setups observed compared control. Germination percentage after 4 days 100 % 65% control (NiS). Similarly, inoculation test enhanced water-use efficiency over 40%. seedlings GGBSU-1 (BiS) showed no signs chlorosis necrosis throughout period against those (PiS) AUH-KAM-9 (KiS). increase chlorophyll-a, chlorophyll-b alpha amylase BiS NiS. Conclusion Inoculating media significantly improved plant. higher due more properties observed.